Polymorphprep, 250 ml
Storage and Stability Conditions of Polymorphprep™
• Polymorphprep™ should be stored at room temperature (20°C).
• It’s stable for 3 years provided the solution is kept sterile and protected from light.
• Prolonged exposure to direct sunlight leads to release of iodine from the Sodium Diatrizoate molecule (this effect is negligible when working with the solution on a day to day basis.)
Polymorphprep is a type of density gradient medium that is used in laboratory settings to separate cells or other biological particles based on their size and density. It is commonly used in a technique called density gradient centrifugation, which involves spinning a tube of Polymorphprep and other components at high speeds to create a gradient of increasing density. This gradient allows cells or other particles to be separated based on their density and size, with heavier or denser particles settling towards the bottom of the tube and lighter or less dense particles remaining towards the top.
Polymorphprep is a specific brand of density gradient medium that is made by Sigma-Aldrich, a company that specializes in supplying chemical, biological, and pharmaceutical products to researchers and other professionals. It is commonly used in a variety of applications, including blood transfusion, tissue culture, and gene expression analysis. Polymorphprep is also used in research and diagnostic laboratories to purify or isolate cells or other biological materials, such as viruses or proteins.
Applications Isolation of human polymorphonuclear cells
Composition The solution contains Sodium Diatrizoate and Dextran 500 in the following concentrations:
• Sodium diatrizoate 13.8% (w/v)
• Dextran 500 8.0 (w/v)
Principle of the separation procedure
How Polymorphprep™ works
Polymorphonuclear leukocytes (PMNs) has a lower buoyant density than erythrocytes (1.09-1.11 g/ml) but a higher buoyant density than the mononuclear cells (higher than 1.085 g/ml). Therefore, it is difficult to isolate polymorphonuclear granulocytes using a density barrier similar to that used for mononuclear cell. Several procedures have been developed to overcomes these issues.
The high osmolality of Polymorphprep™ causes erythrocytes to lose water and shrink, thus increasing their effective buoyant densities. This allows the dextran aggregated erythrocytes to sediment rapidly through the dense medium.
As the cells sediment further into the medium, the osmotic gradient between the medium and the erythrocytes declines. This means that water loss from the erythrocytes is greater at the top of the Polymorphprep™ and progressively decreases as they sediment further. A gradient of diatrizoate is formed within the density barrier. The PMNs band within this density gradient while the mononuclear cells remain at the sample/medium interface.
Polymorphprep™ vs Ficoll-Paque
• Ficoll-Paque (GE Health Care) is not endotoxin tested at a base level and higher price
• Unlike Polymorphprep, Ficoll-Paque is not manufactured under EU cGMP compliance.
Alternatives Polymorphprep™ can be a good substitute for Ficoll-Paque